Journal: Cell reports

Article Title: β-Amyloid Clustering around ASC Fibrils Boosts Its Toxicity in Microglia

doi: 10.1016/j.celrep.2020.02.025

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Thereafter, cells were blocked using 5% normal goat serum (Vector Laboratories) in PTX for 20 min and primary antibodies were added for another 30 min. To check for ASC speck formation, the rabbit anti-ASC (1:250; clone AL177, AdipoGen) or mouse-specific rabbit anti-ASC/TMS1 (1:250, D2W8U, Cell Signaling Technology®) and rat anti-CD11b (1:250; Serotec by Bio-Rad) were used.

Techniques: Control, Recombinant, Magnetic Beads, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay, Quantitation Assay, Gel Extraction, Purification, Isolation, Software, Transmission Assay, Microscopy, Spectrophotometry, Fluorescence, Imaging

Journal: Cells

Article Title: Withaferin A as a Potential Therapeutic Target for the Treatment of Angiotensin II-Induced Cardiac Cachexia

doi: 10.3390/cells13090783

Figure Lengend Snippet: Sequences of primers.

Article Snippet: Antibody used were for NLRP3 (cat MAB7578-SB from R&D Systems), ASC (cat # NBP1-78978 from Novus), and Casp1 (Cat # ab74279), respectively, with appropriate dilution according to instructions from the suppliers.

Techniques:

Journal: Cells

Article Title: Withaferin A as a Potential Therapeutic Target for the Treatment of Angiotensin II-Induced Cardiac Cachexia

doi: 10.3390/cells13090783

Figure Lengend Snippet: Ang II infusion induces activation of NLRP3 inflammasomes. Mice were infused with Ang II as described in Materials and Methods. After 4 weeks of infusion, hearts from each group of animals were collected. The expression levels of NLRP3 ( A ), Casp1 ( B ), and ASC ( C ) were quantified by qRT-PCR. Data are presented as the mean ± SD (n = 5). * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 Ang II-infused vs. saline-infused animals. @@@@ p < 0.0001 Ang II-infused vehicle-treated vs. Ang II-infused WFA-treated animals.

Article Snippet: Antibody used were for NLRP3 (cat MAB7578-SB from R&D Systems), ASC (cat # NBP1-78978 from Novus), and Casp1 (Cat # ab74279), respectively, with appropriate dilution according to instructions from the suppliers.

Techniques: Activation Assay, Expressing, Quantitative RT-PCR, Saline

Journal: Cells

Article Title: Withaferin A as a Potential Therapeutic Target for the Treatment of Angiotensin II-Induced Cardiac Cachexia

doi: 10.3390/cells13090783

Figure Lengend Snippet: Ang II infusion induces expression of NLRP3, ASC, and Caspase 1 proteins. Mice were infused with Ang II as described in Materials and Methods. After 4 weeks of infusion, hearts from each group of animals were collected. The expression levels of NLRP3, ASC, and Caspase 1 proteins in cardiac tissues were evaluated by immunofluorescence technique. Data are presented as the mean ± SD (n = 5). * p < 0.05, *** p < 0.001, and **** p < 0.0001 Ang II-infused vs. saline-infused groups. Scale Bar = 50 µm.

Article Snippet: Antibody used were for NLRP3 (cat MAB7578-SB from R&D Systems), ASC (cat # NBP1-78978 from Novus), and Casp1 (Cat # ab74279), respectively, with appropriate dilution according to instructions from the suppliers.

Techniques: Expressing, Immunofluorescence, Saline